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when-present<#else>when-missing. (These only cover the last step of the expression; to cover the whole expression, use parenthesis: (myOptionalVar.foo)!myDefault, (myOptionalVar.foo)?? ---- ---- FTL stack trace ("~" means nesting-related): - Failed at: ${PackageDetails.images[0]} [in template "templates/web/Test-Package-Page.ftl" at line 414, column 43] ~ Reached through: #nested [in template "templates/web/layout/default-layout.ftl" in macro "default" at line 280, column 10] ~ Reached through: @layout.default [in template "templates/web/Test-Package-Page.ftl" at line 4, column 1] ---- Java stack trace (for programmers): ---- freemarker.core.InvalidReferenceException: [... Exception message was already printed; see it above ...] at freemarker.core.InvalidReferenceException.getInstance(InvalidReferenceException.java:134) at freemarker.core.UnexpectedTypeException.newDescriptionBuilder(UnexpectedTypeException.java:85) at freemarker.core.UnexpectedTypeException.(UnexpectedTypeException.java:48) at freemarker.core.NonHashException.(NonHashException.java:49) at freemarker.core.Dot._eval(Dot.java:48) at freemarker.core.Expression.eval(Expression.java:101) at freemarker.core.DynamicKeyName._eval(DynamicKeyName.java:61) at freemarker.core.Expression.eval(Expression.java:101) at freemarker.core.DollarVariable.calculateInterpolatedStringOrMarkup(DollarVariable.java:100) at freemarker.core.DollarVariable.accept(DollarVariable.java:63) at freemarker.core.Environment.visit(Environment.java:383) at freemarker.core.Environment.invokeNestedContent(Environment.java:633) at freemarker.core.BodyInstruction.accept(BodyInstruction.java:60) at freemarker.core.Environment.visit(Environment.java:383) at freemarker.core.Environment.invokeMacroOrFunctionCommonPart(Environment.java:889) at freemarker.core.Environment.invokeMacro(Environment.java:825) at freemarker.core.UnifiedCall.accept(UnifiedCall.java:84) at freemarker.core.Environment.visit(Environment.java:347) at freemarker.core.Environment.visit(Environment.java:353) at freemarker.core.Environment.process(Environment.java:326) at freemarker.template.Template.process(Template.java:383) at org.springframework.web.servlet.view.freemarker.FreeMarkerView.processTemplate(FreeMarkerView.java:391) at org.springframework.web.servlet.view.freemarker.FreeMarkerView.doRender(FreeMarkerView.java:304) at org.springframework.web.servlet.view.freemarker.FreeMarkerView.renderMergedTemplateModel(FreeMarkerView.java:255) at org.springframework.web.servlet.view.AbstractTemplateView.renderMergedOutputModel(AbstractTemplateView.java:179) at org.springframework.web.servlet.view.AbstractView.render(AbstractView.java:316) at org.craftercms.engine.view.UserAgentAwareCrafterPageView.renderActualView(UserAgentAwareCrafterPageView.java:49) at org.craftercms.engine.view.CrafterPageView.renderMergedOutputModel(CrafterPageView.java:181) at org.springframework.web.servlet.view.AbstractView.render(AbstractView.java:316) at org.springframework.web.servlet.DispatcherServlet.render(DispatcherServlet.java:1404) at org.springframework.web.servlet.DispatcherServlet.processDispatchResult(DispatcherServlet.java:1148) at org.springframework.web.servlet.DispatcherServlet.doDispatch(DispatcherServlet.java:1087) at org.springframework.web.servlet.DispatcherServlet.doService(DispatcherServlet.java:963) at org.springframework.web.servlet.FrameworkServlet.processRequest(FrameworkServlet.java:1006) at org.springframework.web.servlet.FrameworkServlet.doGet(FrameworkServlet.java:898) at javax.servlet.http.HttpServlet.service(HttpServlet.java:655) at org.springframework.web.servlet.FrameworkServlet.service(FrameworkServlet.java:883) at javax.servlet.http.HttpServlet.service(HttpServlet.java:764) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:227) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:162) at org.apache.tomcat.websocket.server.WsFilter.doFilter(WsFilter.java:53) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:189) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:162) at org.craftercms.commons.web.AddResponseHeaderFilter.doFilterInternal(AddResponseHeaderFilter.java:64) at org.springframework.web.filter.OncePerRequestFilter.doFilter(OncePerRequestFilter.java:117) at org.springframework.web.filter.DelegatingFilterProxy.invokeDelegate(DelegatingFilterProxy.java:354) at org.springframework.web.filter.DelegatingFilterProxy.doFilter(DelegatingFilterProxy.java:267) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:189) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:162) at org.craftercms.engine.scripting.impl.ScriptFilter.doFilter(ScriptFilter.java:111) at org.springframework.web.filter.DelegatingFilterProxy.invokeDelegate(DelegatingFilterProxy.java:354) at org.springframework.web.filter.DelegatingFilterProxy.doFilter(DelegatingFilterProxy.java:267) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:189) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:162) at org.springframework.security.web.FilterChainProxy$VirtualFilterChain.doFilter(FilterChainProxy.java:327) at org.springframework.security.web.access.intercept.FilterSecurityInterceptor.invoke(FilterSecurityInterceptor.java:115) at org.springframework.security.web.access.intercept.FilterSecurityInterceptor.doFilter(FilterSecurityInterceptor.java:81) at org.springframework.security.web.FilterChainProxy$VirtualFilterChain.doFilter(FilterChainProxy.java:336) at org.springframework.security.web.access.ExceptionTranslationFilter.doFilter(ExceptionTranslationFilter.java:122) at org.springframework.security.web.access.ExceptionTranslationFilter.doFilter(ExceptionTranslationFilter.java:116) at org.springframework.security.web.FilterChainProxy$VirtualFilterChain.doFilter(FilterChainProxy.java:336) at org.springframework.security.web.session.SessionManagementFilter.doFilter(SessionManagementFilter.java:126) at org.springframework.security.web.session.SessionManagementFilter.doFilter(SessionManagementFilter.java:81) at org.springframework.security.web.FilterChainProxy$VirtualFilterChain.doFilter(FilterChainProxy.java:336) at org.springframework.security.web.servletapi.SecurityContextHolderAwareRequestFilter.doFilter(SecurityContextHolderAwareRequestFilter.java:149) at org.springframework.security.web.FilterChainProxy$VirtualFilterChain.doFilter(FilterChainProxy.java:336) at org.springframework.security.web.savedrequest.RequestCacheAwareFilter.doFilter(RequestCacheAwareFilter.java:63) at org.springframework.security.web.FilterChainProxy$VirtualFilterChain.doFilter(FilterChainProxy.java:336) at org.springframework.security.web.authentication.AnonymousAuthenticationFilter.doFilter(AnonymousAuthenticationFilter.java:109) at org.springframework.security.web.FilterChainProxy$VirtualFilterChain.doFilter(FilterChainProxy.java:336) at org.springframework.security.web.authentication.rememberme.RememberMeAuthenticationFilter.doFilter(RememberMeAuthenticationFilter.java:141) at org.springframework.security.web.authentication.rememberme.RememberMeAuthenticationFilter.doFilter(RememberMeAuthenticationFilter.java:97) at org.springframework.security.web.FilterChainProxy$VirtualFilterChain.doFilter(FilterChainProxy.java:336) at org.springframework.security.web.authentication.AbstractAuthenticationProcessingFilter.doFilter(AbstractAuthenticationProcessingFilter.java:223) at org.springframework.security.web.authentication.AbstractAuthenticationProcessingFilter.doFilter(AbstractAuthenticationProcessingFilter.java:217) at org.springframework.security.web.FilterChainProxy$VirtualFilterChain.doFilter(FilterChainProxy.java:336) at org.springframework.security.web.authentication.logout.LogoutFilter.doFilter(LogoutFilter.java:103) at org.springframework.security.web.authentication.logout.LogoutFilter.doFilter(LogoutFilter.java:89) at org.springframework.security.web.FilterChainProxy$VirtualFilterChain.doFilter(FilterChainProxy.java:336) at org.springframework.security.web.context.SecurityContextPersistenceFilter.doFilter(SecurityContextPersistenceFilter.java:112) at org.springframework.security.web.context.SecurityContextPersistenceFilter.doFilter(SecurityContextPersistenceFilter.java:82) at org.springframework.security.web.FilterChainProxy$VirtualFilterChain.doFilter(FilterChainProxy.java:336) at org.springframework.security.web.FilterChainProxy.doFilterInternal(FilterChainProxy.java:211) at org.springframework.security.web.FilterChainProxy.doFilter(FilterChainProxy.java:183) at org.springframework.web.filter.DelegatingFilterProxy.invokeDelegate(DelegatingFilterProxy.java:354) at org.springframework.web.filter.DelegatingFilterProxy.doFilter(DelegatingFilterProxy.java:267) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:189) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:162) at org.springframework.web.filter.CorsFilter.doFilterInternal(CorsFilter.java:91) at org.springframework.web.filter.OncePerRequestFilter.doFilter(OncePerRequestFilter.java:117) at org.springframework.web.filter.DelegatingFilterProxy.invokeDelegate(DelegatingFilterProxy.java:354) at org.springframework.web.filter.DelegatingFilterProxy.doFilter(DelegatingFilterProxy.java:267) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:189) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:162) at org.tuckey.web.filters.urlrewrite.RuleChain.handleRewrite(RuleChain.java:176) at org.tuckey.web.filters.urlrewrite.RuleChain.doRules(RuleChain.java:145) at org.tuckey.web.filters.urlrewrite.UrlRewriter.processRequest(UrlRewriter.java:92) at org.craftercms.engine.url.rewrite.UrlRewriteFilter.doFilter(UrlRewriteFilter.java:68) at org.springframework.web.filter.DelegatingFilterProxy.invokeDelegate(DelegatingFilterProxy.java:354) at org.springframework.web.filter.DelegatingFilterProxy.doFilter(DelegatingFilterProxy.java:267) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:189) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:162) at org.craftercms.engine.servlet.filter.SiteContextResolvingFilter.doFilter(SiteContextResolvingFilter.java:86) at org.springframework.web.filter.DelegatingFilterProxy.invokeDelegate(DelegatingFilterProxy.java:354) at org.springframework.web.filter.DelegatingFilterProxy.doFilter(DelegatingFilterProxy.java:267) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:189) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:162) at org.craftercms.commons.http.RequestContextBindingFilter.doFilter(RequestContextBindingFilter.java:78) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:189) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:162) at org.apache.logging.log4j.web.Log4jServletFilter.doFilter(Log4jServletFilter.java:64) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:189) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:162) at org.apache.catalina.core.ApplicationDispatcher.invoke(ApplicationDispatcher.java:711) at org.apache.catalina.core.ApplicationDispatcher.processRequest(ApplicationDispatcher.java:459) at org.apache.catalina.core.ApplicationDispatcher.doForward(ApplicationDispatcher.java:385) at org.apache.catalina.core.ApplicationDispatcher.forward(ApplicationDispatcher.java:313) at org.tuckey.web.filters.urlrewrite.NormalRewrittenUrl.doRewrite(NormalRewrittenUrl.java:213) at org.tuckey.web.filters.urlrewrite.RuleChain.handleRewrite(RuleChain.java:171) at org.tuckey.web.filters.urlrewrite.RuleChain.doRules(RuleChain.java:145) at org.tuckey.web.filters.urlrewrite.UrlRewriter.processRequest(UrlRewriter.java:92) at org.craftercms.engine.url.rewrite.UrlRewriteFilter.doFilter(UrlRewriteFilter.java:68) at org.springframework.web.filter.DelegatingFilterProxy.invokeDelegate(DelegatingFilterProxy.java:354) at org.springframework.web.filter.DelegatingFilterProxy.doFilter(DelegatingFilterProxy.java:267) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:189) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:162) at org.springframework.web.filter.OncePerRequestFilter.doFilter(OncePerRequestFilter.java:102) at org.springframework.web.filter.DelegatingFilterProxy.invokeDelegate(DelegatingFilterProxy.java:354) at org.springframework.web.filter.DelegatingFilterProxy.doFilter(DelegatingFilterProxy.java:267) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:189) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:162) at org.craftercms.engine.servlet.filter.ExceptionHandlingFilter.doFilter(ExceptionHandlingFilter.java:55) at org.springframework.web.filter.DelegatingFilterProxy.invokeDelegate(DelegatingFilterProxy.java:354) at org.springframework.web.filter.DelegatingFilterProxy.doFilter(DelegatingFilterProxy.java:267) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:189) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:162) at org.craftercms.engine.servlet.filter.SiteContextResolvingFilter.doFilter(SiteContextResolvingFilter.java:86) at org.springframework.web.filter.DelegatingFilterProxy.invokeDelegate(DelegatingFilterProxy.java:354) at org.springframework.web.filter.DelegatingFilterProxy.doFilter(DelegatingFilterProxy.java:267) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:189) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:162) at org.springframework.web.filter.OncePerRequestFilter.doFilter(OncePerRequestFilter.java:102) at org.springframework.web.filter.DelegatingFilterProxy.invokeDelegate(DelegatingFilterProxy.java:354) at org.springframework.web.filter.DelegatingFilterProxy.doFilter(DelegatingFilterProxy.java:267) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:189) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:162) at org.craftercms.commons.http.RequestContextBindingFilter.doFilter(RequestContextBindingFilter.java:78) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:189) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:162) at org.springframework.web.filter.CharacterEncodingFilter.doFilterInternal(CharacterEncodingFilter.java:201) at org.springframework.web.filter.OncePerRequestFilter.doFilter(OncePerRequestFilter.java:117) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:189) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:162) at org.springframework.web.filter.OncePerRequestFilter.doFilter(OncePerRequestFilter.java:102) at org.springframework.web.filter.DelegatingFilterProxy.invokeDelegate(DelegatingFilterProxy.java:354) at org.springframework.web.filter.DelegatingFilterProxy.doFilter(DelegatingFilterProxy.java:267) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:189) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:162) at org.springframework.web.filter.OncePerRequestFilter.doFilter(OncePerRequestFilter.java:102) at org.springframework.web.filter.DelegatingFilterProxy.invokeDelegate(DelegatingFilterProxy.java:354) at org.springframework.web.filter.DelegatingFilterProxy.doFilter(DelegatingFilterProxy.java:267) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:189) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:162) at org.apache.logging.log4j.web.Log4jServletFilter.doFilter(Log4jServletFilter.java:71) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:189) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:162) at org.apache.catalina.core.StandardWrapperValve.invoke(StandardWrapperValve.java:197) at org.apache.catalina.core.StandardContextValve.invoke(StandardContextValve.java:97) at org.apache.catalina.authenticator.AuthenticatorBase.invoke(AuthenticatorBase.java:541) at org.apache.catalina.core.StandardHostValve.invoke(StandardHostValve.java:135) at org.apache.catalina.valves.ErrorReportValve.invoke(ErrorReportValve.java:92) at org.apache.catalina.valves.AbstractAccessLogValve.invoke(AbstractAccessLogValve.java:687) at org.apache.catalina.core.StandardEngineValve.invoke(StandardEngineValve.java:78) at org.apache.catalina.connector.CoyoteAdapter.service(CoyoteAdapter.java:360) at org.apache.coyote.http11.Http11Processor.service(Http11Processor.java:399) at org.apache.coyote.AbstractProcessorLight.process(AbstractProcessorLight.java:65) at org.apache.coyote.AbstractProtocol$ConnectionHandler.process(AbstractProtocol.java:890) at org.apache.tomcat.util.net.NioEndpoint$SocketProcessor.doRun(NioEndpoint.java:1789) at org.apache.tomcat.util.net.SocketProcessorBase.run(SocketProcessorBase.java:49) at org.apache.tomcat.util.threads.ThreadPoolExecutor.runWorker(ThreadPoolExecutor.java:1191) at org.apache.tomcat.util.threads.ThreadPoolExecutor$Worker.run(ThreadPoolExecutor.java:659) at org.apache.tomcat.util.threads.TaskThread$WrappingRunnable.run(TaskThread.java:61) at java.base/java.lang.Thread.run(Thread.java:829)
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FAQ

What are the reagents used in ZN staining?

The primary stain used in this method is Carbol fuchsin. The decolorizer is an acid alcohol solution. And the secondary stain used is Methylene blue. 

What is the principle behind the acid-fast staining method?

The principle behind the staining of a smear sample is as follows:

  • Carbol fuchsin solubilizes lipoidal material in the Mycobacterial cell wall and penetrates into the cytoplasm with heat, making the cell appear red. 
  • Decolorizing agent is then applied, which removes non-acid fast cells due to their lack of lipoidal material in their cell wall, leaving them colorless. 
  • Acid-fast cells remain red due to the presence of large amounts of lipoidal material in their cell wall that resists the decolorizing solution. 
  • Counterstain, methylene blue, is applied, making decolorized cells absorb its color and appear blue while acid-fast cells retain the red color.

When is the best time for sample collection?

The morning hours are an ideal time to collect sputum samples for testing, as the concentration of bacteria is highest at this point. Doing so can help ensure maximum accuracy in the results obtained.

Why should I book an Acid Fast Stain (ZN Stain, AFB Stain) test with Sterling Accuris?

Sterling Accuris Diagnostics specializes in providing reliable diagnosis support for physicians and other healthcare facilities across the country. It is convenient to book an Acid Fast Stain (ZN Stain, AFB Stain) test in Vadodara  with us at a great price. We also offer safe home collections for your convenience.

Is there any preparation or precautions for patient before tests or body checkup?

Fasting maybe required in case of a few tests to ensure accurate results. 

In general, it is also advisable to abstain from alcohol and heavy diet at least for 24 hours before any health test. You can see the requirement for each test on the product page on our website / app.

Do you provide home visit/collection service?

Yes. We do provide home collection services at your doorstep.

Please go ahead and book your visit today from our top menu options in this website / chat or do call us on 812 813 0000.

How long does it take to receive test results?

Though most tests are completed and reported within about 24 hours of receiving the sample for testing, certain tests take several days to weeks.

Your soft copy will be sent on registered email id and Whatsapp. Also, you will receive SMS with link to download your report. Also, your report is available on our website / app.

To know the status of the report, you can call our customer care team on 812 813 0000.

What makes Sterling Accuris a better pathology lab than others?

For us, precision care, safe sample collection, timely delivery of reports, and precise reporting of patient results while adhering to health and safety precautions are the cornerstone of our success.

What other packages does Sterling Accuris offer?

Sterling Accuris offers a range of packages including Senior citizen (male and female) package, healthy heart profile, diabetes profile, vitamin profile, covid immunity package and much more. Visit our website to learn more about these packages.

Acid Fast Stain (ZN Stain, AFB Stain) Test in Vadodara

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Sterling Accuris Diagnostics offers the Acid Fast Stain (ZN Stain, AFB Stain) Test in Vadodara. This test is also called the Ziehl-Neelsen stain (ZN stain), the hot method of AFB staining, used to identify acid-fast organisms, mainly Mycobacteria.

Overview of Acid Fast Stain (ZN Stain, AFB Stain) Test

Acid-fast staining is a differential staining technique that differentiates acid-fast and non-acid-fast bacteria. Mycobacterium species contain large amounts of mycolic acid in their cell wall. Due to high lipid content, most dyes cannot enter easily through the cell wall, so Mycobacterium species cannot be stained by regular Gram staining. A special staining method is developed to stain such organisms called the Acid-fast staining method. It is also known as the Ziehl-Neelsen method.

For an accurate diagnosis, you can head to Sterling Accuris Diagnostics for an Acid Fast Stain (ZN Stain, AFB Stain) test in Vadodara.

Why and When is an Acid Fast Stain (ZN Stain, AFB Stain) Test Done?

This test is used to:

  1. To identify acid-fast bacilli 
  2. To stain mycobacterium species.

Symptoms:

  • Coughing up blood or mucus
  • Lack of appetite
  • Weight loss
  • Weakness
  • Fatigue
  • Fever and chills
  • Night sweats

Sterling Accuris runs an Acid Fast Stain (ZN Stain, AFB Stain) test in Vadodara that is highly sensitive and provides accurate results. 

Acid Fast Stain (ZN Stain, AFB Stain) Test: Main Highlights 

Instructions: Fasting is not required

Recommended age group: All age groups

Sample type for test: Sputum

How To Interpret Acid Fast Stain (ZN Stain, AFB Stain) Test Report:

  • Acid fast bacilli: 
  • Stains bright red to intensive purple (B), 
  • Straight rod-shaped (sometimes slightly curved)
  • Usually appear as separate cells or in small groups, and may be beaded.
  • Non-acid fast: 
  • Stains Blue color 

Mycobacterial infections can be caused by:

  • Tuberculosis
  • Nontuberculous mycobacteria (NTM) 
  • Pulmonary infections
  • Leprosy 
  • Chronic ulcers (Buruli ulcer)

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